The acoustic backscatter has played a key role in various classification schemes, inputting predictive models and contributing to the interpretation of the marine landscape and its geodiversity. More recently, some studies involving multi-frequency backscatter have begun to be published in the scientific literature, based on the same thinking used in terrestrial remote sensing - that multiple bands allow for greater discrimination of the surface being analyzed. This work, therefore, explores data collected with a multibeam multispectral backscatter echo sounder (frequencies of 170 kHz, 280 kHz, 400 kHz, and 700 kHz) on different seabed types, aiming to understand how the acoustic response behaves according to frequency and seabed type, and to improve seabed classification by applying different analysis approaches and classification models.